NAUFAL, Nur Muhammad (2026) Isolation and Molecular Identification of Thraustochytrid from Mangrove Sediment using 18S rRNA Gene. Undergraduate thesis, Fakultas Sains dan Matematika.

	Text 1. COVER_NUR MUHAMMAD NAUFAL.pdf Download (128kB)
	Text 2. LETTER OF VALIDITY_NUR MUHAMMAD NAUFAL.pdf Download (255kB)
	Text 3. FOREWORD_NUR MUHAMMAD NAUFAL.pdf Download (74kB)
	Text 4. ABSTRACT_NUR MUHAMMAD NAUFAL.pdf Download (131kB)
	Text 5. ABSTRAK_NUR MUHAMMAD NAUFAL.pdf Download (132kB)
	Text 6. TABLE OF CONTENT_NUR MUHAMMAD NAUFAL.pdf Download (108kB)
	Text 9. CHAPTER I INTRODUCTION_NUR MUHAMMAD NAUFAL.pdf Download (165kB)
	Text 14. REFERENCES_NUR MUHAMMAD NAUFAL.pdf Download (200kB)

Abstract

Thraustochytrid are a group of monocentric organisms in the kingdom Chromista
and hold great promise for biotechnological applications. Thraustochytrid are
known to be able to produce polyunsaturated fatty acids, enzymes, polysaccharides,
and secondary metabolites. Mangrove ecosystems in Brebes and Demak exhibit
extensive coverage and hold diverse potential, including the development of fish
hatcheries and ponds and ecotourism. This research aimed to investigate the
diversity of Thraustochytrid present in mangrove sediments in Brebes and Demak
to provide a comprehensive understanding of their genetic variation. The study was
conducted at the Molecular and Applied Microbiology Laboratory, UPT Integrated
Laboratory, Diponegoro University, Semarang, Central Java, and was continued for
4 months. Isolation was performed using the pollen baiting method and glucose,
yeast, and peptone (GYP) media. After isolation, macroscopic and microscopic
identification were conducted, followed by primary screening using the Sudan
Black B method to determine lipid content. Thereafter, molecular identification
using primers 18S001 and 18S13 was performed on isolates that showed the highest
lipid content. A total of four Thraustochytrids (two from Brebes SB, SB1, and two
from Demak SD, SD1) were isolated, and strains SB and SD showed the highest
lipid content. Phylogenetic analysis showed that the SB isolate showed the same
clade as the genus Thraustochytrium, and the SD isolate showed the same clade as
the genus Schyzochytrium. In conclusion, there is a difference in Thraustochytrid
isolates in mangrove sediments from Brebes and Demak.
Keywords: 18S rRNA, mangrove sediment, Thraustochytrid

Actions (login required)

View Item