Syadza, Balqis Feby Sausan (2025) Isolation and Identification of Thraustochytrids Collected from Mangrove Leaves and Sediments at Setiu Wetland, Malaysia. Undergraduate thesis, Fakultas Sains dan Matematika Undip.

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Abstract

Thraustochytrids, a group of heterotrophic protists, predominantly found in
mangrove ecosystems associated with organic material. Thraustochytrids
represents as a valuable source for biotechnological applications since
Thraustochytrids are highly valued for their remarkable polyunsaturated fatty acid
(PUFA) production like DHA, carotenoid, squalene, and hydrolytic enzymes such
as amylase, protease, and cellulase. This study aimed to explore and identify the
genetic diversity of Thraustochytrids from mangrove leaves and sediment within
Setiu Wetland, Malaysia. Isolates were obtained using baiting method, and
characterized through morphological and molecular analysis using
Thraustochytrid-specific primers. Three isolates from decayed mangrove leaves
and one from sediments were obtained. Morphologically, the isolates exhibited
white to orange color, jelly-like to creamy texture, and cellular structures were
sub-globose, aggregated, and granular under microscopic observation. Molecular
analyses revealed that one isolate L332 from Rhizosphora mangrove leaves
belongs to Ulkenia sp., two isolates, L412 and L531 from Aegiceras mangrove
leaves affiliated to Schizochytrium sp., one isolate S421 from sediment showed
close phylogenetic relationships to Botryochytrium sp., while one isolate L532
from Aegiceras mangrove leaves remains unclassified within Thraustochytriaceae
but closely related to Schizochytrium sp. This finding highlights the
underexplored diversity of Thraustochytrids in mangrove ecosystems, also their
morphological variabilities, which support molecular identification and reveals
potential novel lineages.

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