Utomo, Shifa W. (2026) Biocontrol and Growth-Promoting Potential of Endophytic Fungi Isolated from Red Bean (Phaseolus vulgaris) Roots Against Fusarium oxysporum. Undergraduate thesis, Fakultas Sains dan Matematika Undip.

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	Text 3. HALAMAN PENGESAHAN I.pdf Download (139kB)
	Text 4. HALAMAN PENGESAHAN II.pdf Download (472kB)
	Text 6. ABSTRACT.pdf Download (149kB)
	Text 7. DAFTAR ISI.pdf Download (166kB)
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Abstract

Red bean (Phaseolus vulgaris L.) is an important leguminous crop cultivated in tropical and
subtropical regions due to its high nutritional value and economic potential. However, productivity
is often constrained by soil-borne pathogens, Fusarium oxysporum, which causes root rot and
vascular wilt, leading to severe yield losses. Conventional control methods, such as chemical
fungicides, have proven effective but raise concerns regarding environmental safety, human health,
and sustainability. Therefore, biological approaches, including the use of endophytic fungi with
Plant Growth-Promoting Fungi (PGPF) traits, have gained increasing attention as eco-friendly
alternatives. This research was designed to isolate potential fungal endophytes from red bean roots
as biocontrol agents against F. oxysporum as well as their plant growth-promoting properties. The
research methodology involved isolation and purification of fungal endophytes from red bean
roots, macroscopic and microscopic characterization of fungal isolates, antagonism test in dual
culture, PGPF potential test, including phosphate solubilization and indole-3-acetic acid (IAA)
production, and molecular identification using universal ITS primers. Isolation of endophytic fungi
from red bean root tissues; nine isolates, consisting of six isolates identified as Penicillium sp., one
isolate of Fusarium sp., and two isolate of Talaromyces sp. The highest antagonistic activity was
exhibited by four isolates; RBA2, RBA6, RBA7, and RBA11. Isolate RBA7 capable of
solubilizing phosphate and producing indole-3-acetic acid (IAA). Isolate RBA7 was identified as
Talaromyces domesticus, with the highest sequence similarity to Talaromyces domestiucs NRRL
58121, with a percent identity of 99.41%.
Keywords: Phaseolus vulgaris, endophytic fungi, Fusarium oxysporum, biocontrol PGPF.

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