GIBRAN, Raisa Diva (2026) Hematological Profile in Sprague-Dawley RATS (Rattus norvegicus) After Shape Discrimination Treatment. Undergraduate thesis, Fakultas Sains dan Matematika Undip.

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Abstract

This study examines the effect of a shape discrimination test on the hematological
profile of Sprague–Dawley rats (Rattus norvegicus). Research on visual recognition
in rodents predominantly focuses on behavioral outcomes, with limited integration
of physiological markers. Consequently, there is a gap in understanding whether
shape discrimination tasks influence hematological homeostasis, particularly in
widely used laboratory strains such as Sprague–Dawley rats. Sixteen male rats were
subjected to a visual shape discrimination test, after which blood samples were
collected and examined for erythrocyte count, hemoglobin, hematocrit, MCV,
MCH, MCHC, and leukocyte parameters using a hematology analyzer at BBVET
Wates. Descriptive statistical analysis was performed to obtain the mean and
standard deviation of each parameter. The results showed increases in hemoglobin,
hematocrit, MCV, MCH, and MCHC, while leukocyte values remained within
normal physiological ranges. These findings indicate that shape discrimination
induces mild physiological changes without affecting immune function, supporting
its continued relevance as a non-invasive tool in behavioral research.
Keywords: visual cognitive task, behavioral physiology, blood parameter analysis,
rodent hematology, laboratory animal model.

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