Hakim, Sulthan (2024) Isolation and Antibacterial Activity of Lactic Acid Bacteria from Infant Feces Against Food Pathogens Bacteria. Undergraduate thesis, Fakultas Sains dan Matematika Undip.

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Abstract

This study aimed to isolate Lactic Acid Bacteria (LAB) from the feces of
breastfed infants to evaluate their antibacterial activity against pathogenic bacteria.
Ten LAB isolates were successfully obtained and tested using the inhibition zone
method against Staphylococcus aureus and Escherichia coli. The isolation was
performed on MRSA media supplemented with 0.5% CaCO₃, followed by
macroscopic and microscopic characterization. All isolates were confirmed to be
Gram-positive, rod-shaped, and catalase-negative. Antibacterial testing showed that
isolate IF 4 produced the largest inhibition zone against S. aureus with an average
of 13.75 mm, while isolate IF 10 showed an inhibition zone of 10.85 mm against
E. coli. These results indicate that LAB isolates from infant feces exhibit significant
antibacterial activity against pathogenic bacteria, particularly S. aureus and E. coli.
Key words: Antibacterial activity, Escherichia coli, Lactic Acid Bacteria (LAB), Pathogenic bacteria,
Staphylococcus aureus

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