Oktaviana, Hilmy Annisa (2024) Exploring Marine Macroalgae Diversity: rbcL and 18S DNA Barcoding and Phylogenetic Analysis from UMT Beach, Malaysia. Undergraduate thesis, Fakultas Sains dan Matematika Undip.

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Abstract

The precise identification and diversity of marine macroalgae are crucial for comprehending
their ecological functions and uses. This study aims to precisely identify the species of
macroalgae collected from UMT Beach, Kuala Terengganu, Malaysia, using DNA barcoding
procedures. The study uses DNA barcoding techniques to evaluate and clarify the evolutionary
connections among macroalgae species found at University Malaysia Terengganu (UMT)
Beach in Kuala Terengganu, Malaysia. The CTAB method was employed for DNA extraction
in this study, while DNA amplification was carried out using two types of primers, namely 18S
and rbcL. The MEGA program performed the data analysis. The results demonstrated the
successful multiplication of two distinct forms of macroalgae, namely the taxa Cladophora sp.
and Pyropia sp. The findings of this study indicate that the macroalgae species found at UMT
Beach in Kuala Terengganu, Malaysia, are not closely related to each other.
Key words: Marine macroalgae, UMT beach, primer 18S, DNA barcoding, phylogenetic analysis

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