Nurrohmah, Izzah Mardhiyyah (2025) Antibiofilm Activity of Pseudomonas sp. (MB273N) Cell-Free Supernatant against Pathogenic Bacteria. Undergraduate thesis, Fakultas Sains dan Matematika Undip.

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Abstract

Bacterial biofilms have become serious global health concern as they show
resistance against antibiotics as well as the host immune system and are potentially able
to cause infections. Therefore, it is necessary to develop effective strategies to control
biofilm formation. This study aimed to investigate the potency of the cell-free
supernatant (CFS) of Pseudomonas sp. (MB273N) as an antibiofilm agent for
Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and
Chromobacterium violaceum, and to observe the characteristics of bacterial biofilms
with and without treatment of Pseudomonas sp. (MB273N) CFS. The CFS was
prepared by centrifugation of bacterial suspension three times at 11,000 rpm for 15
minutes. A biofilm inhibition assay was performed to assess the antibiofilm activity of
CFS at sub-lethal concentration against four tested bacteria and the cell morphologies
were then observed under Scanning Electron Microscope (SEM). Data analysis used
SPSS version 26. The CFS inhibited biofilm formation, with biofilm inhibition
percentages observed at 14.15% for E. coli, 32.46% for P. aeruginosa, 30.83% for S.
aureus, and 37.5% for C. violaceum. The four bacterial biofilms showed dense cellular
aggregates and were multilayered before CFS treatment. After treatment with the CFS,
the biofilms exhibited less dense, more dispersed biofilm structures, and alteration in
cell morphology. Thus, these findings indicate that Pseudomonas sp. (MB273N) CFS
exhibit antibiofilm activity against E. coli, P. aeruginosa, S. aureus, and C. violaceum.
Keywords: Antibiofilm, Cell-free supernatant, Escherichia coli, Pseudomonas aeruginosa,
Staphylococcus aureus, Chromobacterium violaceum

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