Putri, Rayhan Dhito Arimbi (2025) Effects of Dietary Lacticaseibacillus casei on the Growth Performance, Immune Response, and Disease Resistance Against Aeromonas hydrophila in Nile tilapia (Oreochromis niloticus). Undergraduate thesis, Fakultas Sains dan Matematika Undip.

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Abstract

Nile tilapia (Oreochromis niloticus) is among the most cultivated fish
species globally. However, this species is vulnerable to many bacterial infections,
such as Motile Aeromonas Septicemia (MAS) caused by many species of Aeromonas
bacteria, including Aeromonas hydrophila. This study aimed to evaluate the effects
of Lacticaseibasillus casei feed supplementation on the growth performance,
gastrointestinal colonization, disease resistance, and histopathological changes in
Nile tilapia. For this, 270 Nile tilapia fingerlings were divided into 6 groups, and
in each group, there were 3 replicates of 15 fish. The fish were fed with either
unsupplemented (control) or Lacticaseibasillus casei feed at 106, 107, 108 CFU/g
and supernatant for 1 week before the fish were immersion-challenged with A.
hydrophila at 108 CFU/ml. The fish in each group continued to be fed with their
respective unsupplemented or L casei-supplemented diets for another week, during
which health and mortality were monitored.
The results of this study showed that fish fed with L. casei at 108 CFU/g
showed the highest growth parameters (WG, ADG, SGR, and FCR) although no
statistically significant difference (p>0.05) among the treatment groups. This group
also showed a significantly higher survival rate (p<0.05) post-infection compared
to the positive control and the other treatments, but was not significantly (p>0.05)
from the uninfected control group (negative control). Furthermore, the 108 CFU/g
group showed the highest colonization of LAB in the gastrointestinal tract.
Histopathological observations showed reduced tissue damage in this group
compared to the positive control. This suggests that L. casei may enhance nutrient
absorption and gut colonization, thereby improving immune readiness against
infection. These findings are important as they provide an effective strategy to
enhance fish growth and disease resistance, contributing to more sustainable
practices.

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