WIDYATMIKO, JOHAN YESANTO (2023) STABILITAS PIGMEN DAN ANTIOKSIDAN EKSTRAK THALASSIOSIRA SP. DENGAN PELARUT VIRGIN COCONUT OIL PADA PENYIMPANAN DENGAN SUHU YANG BERBEDA (23dt463). Undergraduate thesis, Fakultas Perikanan dan Ilmu Kelautan.

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Abstract

ABSTRAK
(Johan Yesanto Widyatmiko. Stabilitas Pigmen dan Antioksidan Ekstrak
Thalassiosira sp. dengan Pelarut Virgin Coconut Oil pada Penyimpanan dengan
Suhu yang Berbeda. 26060119130078. Eko Susanto dan Lukita Purnamayati).
Mikroalga Thalassiosira sp. merupakan jenis mikroalga yang memiliki
kandungan senyawa-senyawa bioaktif. Kandunganya antara lain: pigmen klorofil,
karotenoid dan bioaktif lainnya seperti polisakarida, asam lemak tak jenuh.
Ekstraksi pigmen dapat dilakukan dengan menggunakan pelarut hijau seperti Virgin
Coconut Oil. Namun informasi tentang stabilitas pigmen hasil ekstraksi tersebut
belum banyak diketahui. Penelitian ini bertujuan untuk mengkaji pengaruh
penyimpanan dengan suhu berbeda terhadap kualitas pigmen dan antioksidan
ekstrak Thalassiosira sp. dengan pelarut minyak VCO. Ekstraksi dilakukan dengan
suhu 60℃ selama 90 menit. Untuk melihat stabilitasnya, ekstrak Thalassiosira sp.
tersebut disimpan pada suhu 20℃, 40℃, dan 60℃ selama 90 hari. Data uji kadar
pigmen dan antioksidan dianalisis dengan metode rancangan acak kelompok split
plot in time. Parameter pengujian adalah antioksidan, pigmen, dan warna. Data
dianalisis menggunakan analisa ragam (ANOVA). Untuk mengetahui perbedaan
antara perlakuan data diuji dengan uji Beda Nyata Jujur (BNJ). Hasil penelitian
menunjukkan bahwa suhu dan waktu penyimpanan ekstraksi berpengaruh nyata
terhadap hasil nilai kadar pigmen dan antioksidan. Hasil penelitian menunjukkan
bahwa nilai pigmen klorofil a, total klorofil, beta karoten, karotenoid, warna L, dan
antioksidan metode ABTS dan DPPH mengalami penurunan. Namun pada nilai
pigmen pheophytin a, total pheophytin, warna A, warna B, dan warna ΔE
mengalami kenaikan. Suhu dan lama penyimpanan ekstrak mikroalga Thalassiosira
sp. dengan pelarut VCO yang stabil pada penyimpanan 20°C selama 90 hari,
penyimpanan 40°C selama 60 hari, dan penyimpanan 60°C selama 60 hari.
Kata kunci: Mikroalga; Pigmen; Penyimpanan; Suhu; Thalassiosira sp.

ABSTRACT
(Johan Yesanto Widyatmiko. 26060119130078. Stability of Pigments and
Antioxidants of Thalassiosira sp. Extract with Virgin Coconut Oil Solvents at
Different Temperature Storage. Eko Susanto and Lukita Purnamayati).
Microalgae Thalassiosira sp. is a type of microalgae that contains bioactive
compounds. Its contents include: chlorophyll pigments, carotenoids and other
bioactives such as polysaccharides, unsaturated fatty acids. Pigment extraction can
be done using solvents such as Virgin Coconut Oil. However, little information
about the stability of the extracted pigment is known. This study aims to examine
the effect of storage at different temperatures on the quality of pigments and
antioxidants of Thalassiosira sp. with VCO oil solvent. Extraction was carried out
at a temperature of 60℃ for 90 minutes. To see its stability, extract Thalassiosira
sp. These were stored at temperatures of 20℃, 40℃, and 60℃ for 90 days. Pigment
and antioxidant content test data were analyzed using a split plot in time
randomized block design method. Test parameters are antioxidants, pigments and
colors. Data were analyzed using analysis of variance (ANOVA). To determine the
differences between treatments, the data was tested using the Honestly Significant
Difference (BNJ) test. The results showed that the extraction temperature and
storage time had a significant effect on the results of pigment and antioxidant
content values. The results showed that the values of chlorophyll a pigment, total
chlorophyll, beta carotene, carotenoids, L color, and antioxidants in the ABTS and
DPPH methods decreased. However, the values of pigment pheophytin a, total
pheophytin, color A, color B, and color ΔE increased. Temperature and storage
time of Thalassiosira sp microalgae extract. with stable VCO solvent at 20°C
storage for 90 days, 40°C storage for 60 days, and 60°C storage for 60 days.
Keywords: Microalgae; Pigment; Storage; Temperature; Thalassiosira sp.

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