KHAYATI, NURUL (2023) KARAKTERISTIK PIGMEN DAN ANTIOKSIDAN DARI EKSTRAK LIPID Sargassum polycistum DENGAN METODE EKSTRAKSI BERBEDA (23dt462). Undergraduate thesis, Fakultas Perikanan dan Ilmu Kelautan.

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Abstract

ABSTRAK
(Nurul Khayati. 26060119120020. Karakteristik Pigmen dan Antioksidan
dari Ekstrak Lipid Sargassum polycistum dengan Metode Ekstraksi Berbeda.
Eko Susanto dan Eko Nurcahya Dewi).
Rumput laut coklat mengandung lipid sekitar 1-5% dari berat keringnya.
Lipid pada Sargassum polycistum terdiri dari neutral lipids, glikolipids, fosfolipids
dan senyawa bioaktif. Ekstrak lipid Sargassum polycistum dapat diperoleh dengan
modifikasi metode Bligh Dyer, Folch dan Shin menggunakan pelarut organik untuk
memecah dinding sel alga yang mengandung lipid. Tujuan penelitian dilakukan
adalah untuk mengetahui pengaruh metode ekstraksi yang berbeda terhadap
karakteristik pigmen dan antioksidan ekstrak lipid S. polycistum. Metode penelitian
yang dilakukan adalah experimental laboratories dengan Rancangan Acak
Kelompok. Prosedur penelitian ekstraksi lipid yaitu metode Bligh Dyer
perbandingan pelarut akhir yang digunakan kloroform : metanol : air
(1:1:0.9/v:v:v), metode Folch kloroform : metanol : air (2:1:1/v:v:v) dan metode
Shin n-heksan, metanol dan air (1:1:0.9/v:v:v). Penggunaan tiga metode ekstraksi
berpengaruh terhadap total lipid, kandungan pigmen dan aktivitas antioksidan
ekstrak lipid S. polycistum. Hasil total lipid metode Folch 4,82% DW, Bligh Dyer
2,19% DW dan Shin 1,16% DW. Kandungan pigmen klorofil dan turunannya yaitu
1,21-7,15 g/L TL sedangkan golongan karotenoid 0,39-7,93 g/L TL, secara
kualitatif dan karotenoid ekstrak lipid S. polycistum terdeteksi pada panjang
gelombang 300-700 nm, berpotensi sebagai antioksidan. Uji aktivitas antioksidan
metode DPPH dan ABTS menghasilkan nilai IC50 rentang 1,26-2,89 ppm yang
termasuk kategori sangat kuat.
Kata Kunci : Antioksidan, Ekstraksi, Lipid, Pigmen, Sargassum polycistum

ABSTRACT
(Nurul Khayati. 26060119120020. Pigment and Antioxidant
Characteristics of Sargassum polycistum Lipid Extract with Different Extraction
Methods. Eko Susanto and Eko Nurcahya Dewi).
Brown seaweed contains lipids of about 1-5% by dry weight. Lipids in
Sargassum polycistum consist of neutral lipids, glycolipids, phospholipids and
bioactive compounds. Sargassum polycistum lipid extracts can be obtained by
modifying the Bligh Dyer, Folch and Shin methods using organic solvents to break
down algal cell walls containing lipids. The purpose of this study was to determine
the effect of different extraction methods on the pigment and antioxidant
characteristics of S. polycistum lipid extract. The research method used was
experimental laboratories with a randomized group design. Lipid extraction
research procedures are Bligh Dyer method, the final solvent ratio used
chloroform: methanol: water (1:1:0.9/v:v:v), Folch method chloroform: methanol:
water (2:1:1/v:v:v) and Shin method n-hexane, methanol and water (1:1:0.9/v:v:v).
The use of three extraction methods affected the total lipid, pigment content and
antioxidant activity of S. polycistum lipid extract. The total lipid results of Folch
method were 4.82% DW, Bligh Dyer 2.19% DW and Shin 1.16% DW. The content
of chlorophyll pigments and their derivatives is 1.21-7.15 g/L TL while the
carotenoid group is 0.39-7.93 g/L TL, qualitatively and carotenoids of S. polycistum
lipid extract were detected at a wavelength of 300-700 nm, potentially as
antioxidants. Antioxidant activity test of DPPH and ABTS methods resulted in IC50
values ranging from 1.26 to 2.89 ppm which is included in the very strong category.
Keywords: antioxidant, extraction, lipid, pigment, Sargassum polycistum

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