GADALLA, MOHAMED M. Y. and Dharmana, Edi and Kisdjamiatun, Kisdjamiatun (2015) THE EFFECTS OF ANNONA MURICATA LEAVES TOWARDS BLOOD LEVELS OF CXCL9 AND LYMPHOBLAST (STUDY IN CEREBRAL MALARIA PHASE OF SWISS MICE). Masters thesis, Universitas Diponegoro.

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Abstract

Background: Cerebral malaria (CM) forms part of the spectrum of severe malaria, with a case fatality rate ranging from 15% in adults in southeast Asia to 8.5% in children in Africa. Many chemokines have been noted to exhibit increased expression in cerebral malaria, but the chemokines most strongly implicated to have a role are the chemokine (C-X-C motif) receptor 3 (CXCR3) binding chemokines, including chemokine (C-X-C motif) ligand 4 (CXCL4), CXCL9.
Methods:. Swiss albino mice, female , weight 150-200 gram,8-9 week old mice divided into 6 group . K(-) is healthy mice, P1 and P2 were group without inoculated PbA with A. muricata dosage 100 and 150 mg/kg BW. K(+) is group with inoculated PbA, P3 and P4 were group with inoculated PbA and A. muricata dosage 100 and 150 mg/kg BW. Take the blood from mice’s eye after mice anaesthezed. Make the spleen culture with stimulate CXCL9, incubation 72 hours and then collect the cell to efipendorf tube, spin the tube with microcentrifuge. Keep the sample to the refrigerator on -80°C to analyze the result and lymphoblast by staining with giemsa and count the percentage 100 cells .data analyze with ANOVA using SPSS. Results: the normality test the data is normal because p-value higher than 0.05 and CXCL 9 in all dose studied had no association with the lymphoblast in healthy swiss mice. Conclusion: A. muricata not significant reduce the level of spleen CXCL9 in Plasmodium berghei ANKA infected mice. A. muricata not significant increase the level of lymphoblast in Plasmodium berghei ANKA infected mice.
Keywords: CXCL9, PbA-infected mice, A. muricata, Lymphoblast level.

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