PUTRI, Ni Made Marlin Suarjo and Sutiningsih, Dwi and Hadi, Mochamad (2022) EFEKTIVITAS HAND SANITIZER SPRAY GEL NANOPARTIKEL PERAK EKSTRAK DAUN PEPAYA (Carica papaya L) SEBAGAI ANTIBAKTERI. Masters thesis, UNIVERSITAS DIPONEGORO.

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Abstract

Latar Belakang: Praktek mencuci tangan sebagai salah satu cara pencegahan infeksi. Penggunaan sanitizer berbahan alkohol pada jangka panjang dapat membuat iritasi pada kulit. Oleh sebab itu, diperlukan sanitizer berbahan alami. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri dan keamanan nanopartikel perak ekstrak daun pepaya sebagai hand sanitizer spray gel.
Metode: Jenis penelitian true experiment dengan Post-test only with control group design. Kombinasi daun pepaya dengan nanopartikel perak kosentrasi 100 ppm, 150 ppm, 200 ppm, 250 ppm diujikan pada bakteri Staphylococcus epidermidis dan Salmonella typhi dengan metode difusi (Kirby bauer). Kosentrasi yang digunakan ialah 250 ppm dan akan diformulasikan menjadi hand sanitizer serta diuji iritasi. Uji iritasi menggunakan kelinci sebanyak 27 ekor. Sebelum pengujian kelinci di aklimitasi 7 hari, setelah itu pada daerah punggung di cukur seluas 4x6 cm dan diolesi sediaan sanitizer.
Hasil: Hasil penelitian menunjukkan kosentrasi ekstrak nanopartikel perak daun pepaya mulai dari 150, 200, 250 ppm berpotensi membunuh bakteri Staphylococcus epidermidis dan Salmonella typhi. Berdasarkan perhitungan pemeriksaan indeks iritasi (PII) kosentrasi 250 ppm nanopartikel perak ekstrak daun pepaya pada waktu 24, 48 dan 72 jam menunjukkan adanya sedikit kemerahan pada kulit (eritema) dengan skor 0,11 (negligible) dan tidak menunjukkan adanya pembengkakkan kulit (Endema).
Kesimpulan: Ekstrak nanopartikel perak daun pepaya dapat berpotensi sebagai hand sanitizer alami yang dapat membunuh bakteri dengan tingkat iritasi tergolong rendah.
Keyword: Daun Pepaya, Nanopartikel perak, Hand Sanitizer

Background: The practice of washing hands as one of the ways to prevent infection. Long-term use of alcohol-based sanitizers can irritate the skin. Therefore, a natural sanitizer is needed. This study aims to determine the antibacterial activity and safety of silver nanoparticles of papaya leaf extract as a hand sanitizer spray gel.
Method: Type of true experiment research with Post-test only with control group design. The combination of papaya leaves with silver nanoparticles of concentration of 100 ppm, 150 ppm, 200 ppm, 250 ppm was tested on Staphylococcus epidermidis and Salmonella typhi bacteria by diffusion method (Kirby bauer). The concentration used is 250 ppm and will be formulated into hand sanitizer and tested for irritations. The test itself used 27 rabbits, Before the test run the rabbits have to be accremated for 7 days, after that the rabbits got shaved on the back area of 4x6 cm and smeared with sanitizer preparations.
Results: The results showed that the concentration of silver nanoparticle extracts of papaya leaves ranging from 150, 200, 250 ppm has the potential to kill Staphylococcus epidermidis and Salmonella typhi bacteria. Based on the calculation of irritation index (PII) examination, the concentration of 250 ppm silver nanoparticles of papaya leaf extract at 24, 48 and 72 hours showed a slight redness of the skin (erythema) with a score of 0.11 (negligible) and did not show any skin bender (Endema).
Conclusion: Papaya leaf silver nanoparticle extract can potentially be a natural hand sanitizer that can kill bacteria with relatively low levels of irritation.
Keyword: Papaya Leaf, Silver Nanoparticles, Hand Sanitizer

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