TAMA, HEKA RIA (2022) EFEK PEMBERIAN ETHINYLESTRADIOL DOSIS BERBEDA TERHADAP PERFORMA REPRODUKSI INDUK LELE (Clarias gariepinus Burchell) BETINA (22b116). Undergraduate thesis, Universitas Diponegoro.

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Abstract

RINGKASAN
Heka Ria Tama. 26020118140056. Efek Pemberian Ethinylestradiol Dosis
Berbeda Terhadap Performa Reproduksi Induk Lele (Clarias gariepinus Burchell)
Betina (Fajar Basuki dan Tristiana Yuniarti)
Proses induk lele (Clarias gariepinus Burchell) betina menjadi matang
gonad secara alami memakan waktu hingga 2-3 bulan, dengan tingginya permintaan
benih lele diperlukan percepatan proses pematangan gonad agar permintaan benih
dapat terpenuhi, salah satunya melalui pemberian hormon ethinylestradiol.
Kandungan hormon optimum ethinylestradiol pada tubuh induk lele betina akan
mempercepat proses vitellogenesis sehingga memacu pertumbuhan oosit dan
mempercepat kematangan gonad. Penelitian ini bertujuan untuk mengetahui
pengaruh penyuntikan hormon ethinylestradiol dosis berbeda terhadap performa
reproduksi dan mengetahui dosis terbaik terhadap kecepatan pematangan gonad
induk lele betina. Penelitian ini dilakukan tanggal 11 Januari – 25 Februari di
Teaching Factory Undip. Ikan uji menggunakan induk lele betina dengan bobot 1-1,1 kg. Penelitian ini menggunakan metode eksperimental dengan rancangan acak
lengkap (RAL) terdiri dari 4 perlakuan dan 3 ulangan yakni perlakuan A (minyak
jagung 0,2 ml/kg induk), perlakuan B (0,3 ml ethinylestradiol/kg induk), perlakuan
C (0,5 ml ethinylestradiol/kg induk), dan perlakuan D (0,7 ml ethinylestradiol/kg
induk). Data yang diamati yaitu kecepatan pematangan gonad, histologi gonad,
fekunditas, derajat pembuahan, derajat penetasan, derajat kelulushidupan dan
kualitas air. Hasil penelitian menunjukkan dosis terbaik yaitu perlakuan D (0,7 ml
ethinylestradiol/kg) dengan kecepatan pematangan gonad selama 22 ± 1 hari,
pengamatan histologi menunjukan matang gonad di TKG IV, nilai fekunditas
47.600 ± 2424, derajat pembuahan (FR) sebesar 88,60 ± 1,52%, derajat penetasan
(HR) sebesar 82,50 ± 1,80%, dan derajat kelulushidupan (SR) 84,50 ± 1,32%. Hasil
pengukuran kualitas air variabel suhu adalah 24,1- 27,5ºC, DO 3,27 - 4,37 ppm dan
pH 7,34 – 8,43. Hasil penelitian menunjukan penyuntikan ethinylestradiol
berpengaruh nyata terhadap performa reproduksi induk lele betina dengan
perlakuan D (0,7 ml ethinylestradiol/kg induk) sebagai dosis terbaiknya.
Kata kunci : Estradiol, hormon, kematangan gonad, reproduksi, vitellogenesis

SUMMARY
Heka Ria Tama. 26020118140056. The Effects of Ethinylestradiol with
Different Doses on Reproductive Performance of Female Catfish (Clarias
gariepinus Burchell) Broodstock (Fajar Basuki and Tristiana Yuniarti)
The maturing gonads process of female catfish (Clarias gariepinus
Burchell) naturally takes up to 2-3 months, it is necessary to accelerate the
gonadal maturation process so that the demand for catfish seeds can be met
through the injection of the ethinylestradiol hormone. The optimum hormone
content of ethinylestradiol in the body of catfish broodstock will accelerate the
vitellogenesis process so that it stimulates oocyte growth and gonads can mature
more quickly. This study aimed to determine the best dose of ethinylestradiol
injection and its effect on the reproductive performance of female broodstock
catfish. This research was conducted on 11 January – 25 February at the Teaching
Factory Undip. The test fish used female catfish with a weight of 1-1.1 kg. This
study used an experimental method with a completely randomized design (CRD)
consisting of 4 treatments and 3 replications, namely treatment A (corn oil 0.2
ml/kg broodstock), treatment B (0.3 ml ethinylestradiol/kg broodstock), treatment
C (0.5 ml ethinylestradiol/kg broodstock), and treatment D (0.7 ml
ethinylestradiol/kg broodstock). The data observed were gonadal maturation time,
gonadal histology, fecundity, fertilization rate, hatching rate, survival rate, and
water quality. The results showed that injection of ethinylestradiol had a
significant effect on the reproductive performance of female catfish and the best
dose was obtained namely treatment D (0.7 ml ethinylestradiol/kg) with the speed
of gonadal maturation was 22 ± 1 days, histological observations showed gonadal
maturity level IV, fecundity 47,600 ± 2424, fertilization rate (FR) 88.60 ± 1.52%,
hatching rate (HR) 82.50 ± 1.80%, and survival rate (SR) 84.50 ± 1.32%. The
results of the measurement of water quality variable temperature are 24.1- 27.5
o
C,
Dissolved Oxygen 3.27 – 4.37 ppm and pH 7.34 – 8.43. The results showed that the
injection of ethinylestradiol had a significant effect on the reproductive
performance of female catfish with treatment D (0.7 ml ethinylestradiol/kg) as the
best dose.
Keywords: Estradiol, gonadal maturation, hormone, reproduction, vitellogenesis

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