Waridiarto, Dimas and Prajoko, Yan Wisnu and Prihharsanti, Nawangsih (2021) PENGARUH PEMBERIAN ALOE VERA TEROZONASI TOPIKAL TERHADAP EKSPRESI TNF-α DAN RASIO NEUTROFIL LIMFOSIT PADA DERMATITIS RADIASI TIKUS SPRAGUE DAWLEY. Masters thesis, Universitas Diponegoro.

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Abstract

Radiation dermatitis is a side effect is the most common side effect of external radiation with varied clinical features. Applying topical ozonated aloe vera can reduce the inflammatory reaction in radiation dermatitis by decreasing neutrophil migration and TNF-α expression. This study aims to improve the healing response of radiation dermatitis with ozonated aloe vera therapy based on TNF-α face and the ratio of neutrophils to lymphocytes. Experimental study with post-test only randomized control group design on 36 male Sprague Dawley rats weighing 150±50 grams and given a single dose of 7 Gy radiation. After radiation, acclimatization was carried out for seven days and followed by randomization and divided into six groups, namely negative control, positive control given hydrocortisone cream 2.5%, treatment groups 1, 2, 3, and 4 were given aloe vera, aloe vera, respectively ozonated doses of 300 mg/mL, 600 mg/ml, and 1200 mg/mL for seven days. After the treatment, the rats were terminated, and histological preparations were made with HE staining and immunohistochemistry to see the NL ratio and TNF-α. Three rats were dead on external radiation administration. In the Kruskal-Wallis test, there were significant differences in the NL ratio and TNF-α expression based on the type of treatment (p <0.05). In the Mann-Whitney test, there were significant differences between treatment groups (p < 0.05). Ozonated aloe vera increases radiation dermatitis's healing response in Sprague Dawley rats by decreasing TNF-α expression and neutrophil-lymphocyte ratio.

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