GIRSANG, PUTRI TAYUVANI (2025) THE EFFECT OF BAP AND AMBON BANANA EXTRACT ON THE GROWTH OF CATTLEYA ORCHIDS (Sureeya Gold × Ken Arok) IN SUB-CULTURE II TISSUE CULTURE. Undergraduate thesis, Fakultas Peternakan dan Pertanian Universitas Diponegoro.

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Abstract

The research aims to examine the effect of administering Benzyl Amino
Purine (BAP) And extract banana on growth orchid cattleya ( Sureeya Gold × Ken
Arok ) on sub culture II in a way culture network. Study has implemented on
November 13, 2024 – February 11, 2025 at the Tissue Culture Laboratory, Faculty
of Animal Husbandry and Agriculture, Diponegoro University, Semarang.
The research method used a 4 x 4 factorial experiment using a Completely
Randomized Design (CRD) with 5 replications used in the research, so that there
is 80 unit unit test. Factor First is BAP with 4 levels, namely 0 ppm (control), 1.5
ppm, 3.0 ppm, 4.5 ppm. The second factor is Ambon banana extract with 4 levels,
namely 0 g/L (control), 10 g/L, 20 g/L, 30 g/L. The parameters observed include
the time of shoot emergence, the percentage of browning explants, percentage
explanation contaminated, amount shoots And, amount leaf. The data obtained
were analyzed using variance and further Honestly Significant Difference (HSD)
tests.
The results of the study showed that the administration of BAP significantly
affected the growth of shoot emergence time and number of leaves, namely the 4.5
ppm BAP treatment which produced the fastest shoot emergence time and 0 ppm
BAP which produced a greater number of leaves, the banana extract treatment at
the 20 g/L level provided the fastest shoot emergence time and number of shoots,
the interaction of 1.5 ppm BAP treatment on 10 g/L banana extract was the
interaction that produced the best shoot emergence time, the interaction of 0 ppm
BAP and 30 g/L banana extract produced the best number of leaves. The highest
percentage of browning explant media was 40% each 2 bottle Which browning on
treatment B0P0 And B1P1. The highest percentage of contaminated explants was
80% in the B0P0 treatment, with 4 bottles contaminated by fungi. Based on the
research that has been done, it can be concluded that the optimal growth of
cattleya orchid explants in PLB growth is the BAP treatment at a level of 4.5 ppm,
and Banana Extract at a level of 20 g/L and there is an interaction between the two
factors, BAP and Banana Extract.

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