HASUGIAN, KEZIA WINONA (2025) THE EFFECT OF Indole-3-Butyric AC ID (IBA) CONCENTRATION AND CUTTING LENGTH ON THE PROPAGATION AND GROWTH OF GRAPE (Vitis vinifera L.). Undergraduate thesis, Fakultas Peternakan dan Pertanian Universitas Diponegoro.

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Abstract

This study aims to determine the effect of different concentrations of Indole
3-Butyric Acid (IBA) and cutting stem lengths on the success of propagation and
growth of grape plants ( Vitis vinifera L.). This study was conducted in the
greenhouse of SMK Negeri H. Moenadi Ungaran and the Physiology Laboratory.
And Plant Breeding, Faculty Animal and Agricultural Sciences, Universitas
Diponegoro on December 2, 2024 – March 17, 2025.
The experimental design used was Factorial with 2 factors and 3 test. Factor
First is giving concentration auxin synthetic IBA with 5 level that is control or 0
ppm (Z0) , 100 ppm (Z1) , 150 ppm (Z2) , 200 ppm (Z3) , and 250 ppm (Z4) . The
second factor was the length of the stem cuttings with 3 treatment levels, namely
15 cm (P1), 20 cm (P2), and 25 cm (P3). The design produced 15 combinations.
And 45 unit test with every unit there is 5 stem cuttings This resulted in a total of
225 cuttings. Observed parameters included shoot emergence time, shoot number,
shoot length, leaf number, root number, root length, total chlorophyll, and the
percentage of live cuttings.
The results showed that soaking the cuttings in IBA solution significantly
affected the parameters of the number of shoots, shoot length, number of leaves,
number of roots, root length, and total chlorophyll. The length of the cuttings
significantly affected the parameters of the time of shoot emergence and the
percentage of live cuttings. The interaction between the soaking solution and the
length of the cuttings significantly affected the length of shoots, root length, and
total chlorophyll. The concentration of 200 ppm show best results on all over
Parameters. A stem length of 15 cm indicates the fastest shoot emergence time.
The interaction between a 200 ppm IBA soaking solution and a 20 cm stem length
increased shoot and root length, as well as total chlorophyll in the leaves.
The conclusion of this study is that the IBA concentration of 200 ppm (Z3)
showed the best results in cutting growth. Cuttings with a stem length of 15 cm
(P1) showed the fastest shoot emergence time. Soaking cuttings in a solution with
a concentration of 200 ppm (Z3) with a cutting length of 20 cm (P2) resulted in
the longest average shoot and root length, as well as the highest total chlorophyll.

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