WIDYANINGRUM, ASIH (2024) Identification of Pathogens Causing Leaf Blight Disease in Corn Plants. Undergraduate thesis, Fakultas Peternakan dan Pertanian Universitas Diponegoro.

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Abstract

This study aims to identify the pathogens causing corn leaf blight disease in
several corn-producing regions in Central Java, namely Grobogan, Semarang, and
Klaten. The research was conducted from January 2024 to June 2024 at the
Laboratory of Plant Physiology and Breeding, Faculty of Animal Husbandry and
Agriculture, Diponegoro University, Semarang, and on land in Pucung Village,
Bancak District, Semarang Regency, Central Java.
The research was divided into several stages, namely exploration of downy
mildew symptoms, identification of downy mildew morphology and morphometry,
molecular identification of downy mildew, and inoculation of downy mildew
pathogens. Exploration of downy mildew symptoms was carried out using a
purposive sample method based on the intensity of attacks in three locations,
namely Grobogan, Semarang, and Klaten, which are among the corn-producing
centers in Central Java. Morphological and morphometric identification was
performed by microscopically observing the shape and size of the spores. Molecular
identification was performed using specific primers, namely PmUR/PmUF specific
to the species Peronosclerospora maydis, PpUR/PpUF specific to the species
Pernosclerspora philippinensis, and PsrUR/PsrUF specific to the species
Peronosclerospora sorghi. Pathogen inoculation was carried out on Paramita F1
corn plants using a monofactor experiment and a completely randomized design
(CRD). The experiment used inoculum sources from Grobogan, Semarang, and
Klaten that had been molecularly identified as Peronosclerospora sorghi. The
research environment had an average temperature of 31.9°C and an average
humidity of 76%. Pathogen inoculation was carried out using the infected leaf
insertion method, which involved inserting symptomatic leaf inoculum sources
between test corn plants that were 1 day old after planting (DAP).

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