MARHAENY, ANNISA (2023) AKTIVITAS ANTIBAKTERI EKSTRAK METANOL RUMPUT LAUT Kappaphycus alvarezii (Doty) Doty, 1988 TERHADAP Staphylococcus aureus DAN Escherichia coli (23dik434). Undergraduate thesis, Fakultas Perikanan dan Ilmu Kelautan.

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Abstract

ABSTRAK
(Annisa Marhaeny. 26040119130091. Aktivitas Antibakteri Ekstrak
Metanol Rumput Laut Kappaphycus alvarezii (Doty) Doty, 1988 Terhadap
Staphylococcus aureus dan Escherichia coli. Rini Pramesti dan Suryono).
Bakteri yang menyebabkan penyakit pada inangnya disebut patogen. Penelitian ini
bertujuan mengetahui senyawa bioaktif dan aktivitas antibakteri yang terkandung
dalam ekstrak metanol K. alvarezii. Penelitian dilakukan dengan metode
deskriptif eksploratif. Bakteri yang diujikan yaitu Staphylococcus aureus dan
Escherichia coli. Ekstraksi dilanjutkan dengan uji fitokimia. Ekstrak diencerkan
menggunakan dimetil sulfoksida (DMSO) hingga didapatkan konsentrasi uji 500
µg/disk, 1000 µg/disk, dan 1500 µg/disk. Cefadroxil dan DMSO digunakan
sebagai kontrol positif serta negatif. Uji aktivitas antibakteri digunakan dengan
metode difusi cakram. Inokulum diinkubasi selama 3x24 jam pada suhu 37ºC.
Zona hambat dihitung setiap 24 jam sekali. Hasil uji aktivitas antibakteri ekstrak
metanol K. alvarezii terhadap bakteri S. aureus dan E. coli menunjukkan zona
hambat terbesar pada konsentrasi 500 µg/disk di waktu 48 jam sebesar 4,95 mm
dan 5,1 mm. Senyawa bioaktif yang terkandung dalam ekstrak metanol K.
alvarezii yaitu senyawa alkaloid, flavonoid, tanin, dan steroid.
Kata kunci: antimikroba, makroalga, senyawa bioaktif, uji fitokimia

ABSTRACT
(Annisa Marhaeny. 26040119130091. Antibacterial Activity of Methanol
Extract of Seaweed Kappaphycus alvarezii (Doty) Doty, 1988 Against
Staphylococcus aureus and Escherichia coli. Rini Pramesti and Suryono).
Bacteria that cause disease in their hosts are called pathogens. This study aims to
determine the bioactive compounds and antibacterial activity in the methanol
extract of K. alvarezii. The research was conducted using a exploratory
descriptive method. The bacteria tested were Staphylococcus aureus and
Escherichia coli. Extraction then phytochemical tests were carried out The extract
was diluted using dimethyl sulfoxide (DMSO) to obtain test concentrations of 500
µg/disk, 1000 µg/disk, and 1500 µg/disk. Cefadroxil and DMSO were used as
positive and negative controls. Antibacterial activity test using disc diffusion
method. The inoculum was incubated for 3x24 hours at 37°C. The inhibition zone
is calculated every 24 hours. The results of the antibacterial activity test of K.
alvarezii methanol extract against S. aureus and E. coli bacteria showed the
largest inhibition zone at a concentration of 500 µg/disk in 48 hours of 4.95 mm
and 5.1 mm. The bioactive compounds contained in the methanol extract of K.
alvarezii are alkaloids, flavonoids, tannins, and steroids.
Keyword: antimicrobial, bioactive compounds, macroalgae, phytochemical test

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