RAHAYU, SRI MUGI (2023) PENGARUH MINYAK POHON TEH (Melaleuca alternifolia) SEBAGAI BAHAN ANESTESI TERHADAP KADAR GLUKOSA DARAH DAN KELULUSHIDUPAN BENIH IKAN TAWES (Barbonymus gonionotus) PADA TRANSPORTASI SISTEM TERTUTUP (23d389). Undergraduate thesis, Fakultas Perikanan dan Ilmu Kelautan.

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Abstract

ABSTRAK
(Sri Mugi Rahayu. 26020119130124. Pengaruh Minyak Pohon Teh (Melaleuca alternifolia) sebagai Bahan Anestesi terhadap Kadar Glukosa Darah dan Kelulushidupan Benih Ikan Tawes (Barbonymus gonionotus) pada Transportasi Sistem Tertutup. Desrina dan Dicky Harwanto).
Transportasi benih menjadi bagian penting untuk mendukung suksesnya budidaya ikan tawes. Rendahnya kelulushidupan ikan sebesar 30-40% menjadi permasalahan yang kerap dihadapi oleh pembudidaya dalam proses transportasi ikan tawes. Penambahan minyak pohon teh sebagai bahan anestesi diharapkan dapat meminimalisasi kematian ikan dengan menurunkan aktivitas metabolisme karena hasil metabolisme ikan yang tinggi dapat menyebabkan ikan menjadi stres bahkan mati. Penelitian ini bertujuan untuk mengetahui pengaruh minyak pohon teh terhadap waktu induksi, waktu recovery, kadar glukosa darah dan kelulushidupan benih ikan tawes serta mengetahui dosis terbaik minyak pohon teh untuk anestesi benih ikan tawes. Penelitian ini dilaksanakan di Balai Budidaya Ikan Air Tawar (BBIAT) Muntilan, Magelang. Penelitian ini menggunakan metode eksperimental dengan rancangan acak lengkap (RAL) yang terdiri dari 4 perlakuan dan 3 ulangan. Perlakuan yang diberikan adalah minyak pohon teh dosis 0,00 mL/L; 0,10 mL/L; 0,15 mL/L dan 0,20 mL/L air. Ikan uji yang digunakan adalah benih ikan tawes berukuran 5-7 cm pada kepadatan 22 ekor/L air dalam kantong packing. Prosedur penelitian dimulai dengan aklimatisasi ikan selama 2 hari, pemuasaan selama 1 hari kemudian pengukuran glukosa darah sebelum transportasi. Bahan anestesi dilakukan uji fitokimia. Ikan dikemas dalam kantong packing, ditambahkan bahan anestesi serta diamati waktu induksi dan tingkah laku ikan. Transportasi ikan selama 10 jam dan setelah transportasi dilakukan pengukuran glukosa darah, kualitas air serta pengamatan waktu recovery dan kelulushidupan ikan. Ikan dipelihara selama 7 hari serta dilakukan pengukuran kualitas air dan kelulushidupan ikan. Dosis terbaik minyak pohon teh sebagai bahan anestesi untuk transportasi benih ikan tawes adalah 0,10 mL/L yang menghasilkan kelulushidupan ikan setelah transportasi sebesar 86,33±4,51%. Waktu induksi yaitu 22,36±0,80 menit. Waktu recovery yaitu 2,58±0,45 menit. Glukosa darah ikan setelah transportasi yaitu 71,89±3,24 mg/dL.
Kata Kunci: Anestesi, Glukosa Darah, Kelulushidupan, Tawes, Transportasi

ABSTRACT
(Sri Mugi Rahayu. 26020119130124. The Effect of Tea Tree Oil (Melaleuca alternifolia) as Anesthethic on Blood Glucose Level and Survival Rate of Java Barb (Barbonymus gonionotus) Seeds in Closed Transportation System. Desrina and Dicky Harwanto).
Seed transportation is an important part of supporting the success of java barb cultivation. The low survival of fish by 30-40% is a problem that is often faced by cultivators in the process of java barb transportation. The addition of tea tree oil as an anesthetic agent is expected to minimize fish mortality by reducing metabolic activity because high fish metabolism results can cause fish to become stressed and even die. This study aims to determine the effect of tea tree oil on induction time, recovery time, blood glucose levels and survival of java barb seeds and to determine the best dose of tea tree oil for java barb seed anesthesia. The research was conducted at Freshwater Fish Cultivation Hall Muntilan, Magelang. This study used an experiment method with a completely randomized design with 4 treatments and 3 replications. The treatment given was tea tree oil dose of 0,00 mL/L; 0,10mL/L; 0,15 mL/L and 0,20 mL/L water. The test fish used were java barb seeds measuring 5-7 cm and a density of 22 fish/L of water in a packing bag. The research procedure began with fish acclimatization for 2 days, fasting for 1 day the blood glucose measurement before transportation. The fish were packed in packing bag, added anesthetic agent and observed the induction time and behavior of the fish. The anesthetic agent was tested for phytochemicals. Transportation of fish for 10 hours and after transportation, blood glucose measurements, water quality and observation of recovery time and fish survival. Fish were reared for 7 days and measurement of water quality and fish survival. The results showed that tea tree oil had a significant effect on induction time, recovery time, fish blood glucose after transportation and fish survival. The best dose of tea tree oil as an anesthetic for transportation of java barb seeds is 0,10 mL/L which results in fish survival after transportation of 86,33±4,51%. Induction time is 22,36±0,80 minutes. Recovery time is 2,58±0,45 minutes. Fish blood glucose after transportation was 71,89±3,24 mg/dL.
Keywords: Anesthetic, Blood Glucose, Java Barb, Survival Rate, Transportation

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