WIRAYUDHA, ADITYA DWIPUTRA (2023) PENGARUH PERBEDAAN MEDIA KULTUR TERHADAP AKTIVITAS ANTIMIKROBA KAPANG Gloniopsis leucaenae ASOSIASI Rhizophora mucronata (23dik173). Undergraduate thesis, Fakultas Perikanan dan Ilmu Kelautan.

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Abstract

ABSTRAK
(Aditya Dwiputra Wirayudha. 26040119130052. Pengaruh Perbedaan
Media Kultur Terhadap Aktivitas Antimikroba Kapang Gloniopsis leucaenae
Asosiasi Rhizophora mucronata Wilis Ari Setyati dan Ali Ridlo)
Jamur diketahui menghasilkan metabolit sekunder dan memiliki aktivitas
antimikroba. Beberapa faktor mempengaruhi produksi metabolit sekunder pada
jamur, termasuk media kultur. Penelitian ini bertujuan untuk menentukan pengaruh
media kultur yang berbeda pada aktivitas antimikroba dari Gloniopsis leucaenae
yang berasosiasi dengan Rhizophora mucronata dan mengidentifikasi senyawasenyawa yang bertindak sebagai antimikroba. Strain G. leucaenae (MT.F.27.M)
dibiakkan dalam air tawar dan air asin dari media masing-masing Potato Dextrose
Agar (PDA), Malt Extract Agar (MEA), Potato Dextrose Broth (PDB), Malt
Extract Broth (MEB). Metabolit yang berada di miselium dan agar diekstraksi
menggunakan metanol sedangkan metabolit yang berada di dalam kaldu (Broth)
diekstraksi menggunakan etil asetat. Ekstrak kasar diuji antimikroba terhadap M.
luteus, B. subtillis, P. aeruginosa, C. albicans, dan C. tropicals. Sementara itu,
metabolit jamur dikarakterisasi dengan uji fitokimia, Thin Layer Chromatography
(TLC), dan Gas Chromatography-Mass Spectrometer (GC-MS). Uji aktivitas
antimikroba menunjukkan bahwa PBt-M paling efektif sebagai antimikroba
terhadap Micrococcus luteus ( Minimum Inhibition Concentration (MIC) 6,25
µg/ml; Minimum Bactericidal Concentration (MBC) 12,5 µg/mL) dan Bacillus
subtilis (MIC 1,56 µg/mL; MBC 1,56 µg/mL). Karakterisasi metabolit untuk
ekstrak PBt-M menggunakan tes fitokimia dan analisis TLC menunjukkan
keberadaan Alkaloid dan Fenol. Sementara itu, hasil analisis GC-MS berhasil
mengungkapkan keberadaan senyawa seperti Trilaurin; vinil laurat; dan
Dodecanamide, N-dodecyl-N-(trifluoroacetyl).
Kata Kunci: antibakteri; Gloniopsis; jamur; mangrove

ABSTRACT
(Aditya Dwiputra W. 26040119130052. The Effect of Culture Media on
Antimicrobial Activity of Mold Gloniopsis leucaenae Associated Rhizophora
mucronata Wilis Ari Setyati dan Ali Ridlo)
Fungi are known to produce secondary metabolites and have antimicrobial
activity. Several factors influence the production of secondary metabolites in fungi,
including culture media. This study aims to determine the effect of different culture
media on the antimicrobial activity of Gloniopsis leucaenae associated with
Rhizophora mucronata and to identify compounds that act as antimicrobials. G.
leucaenae strain (MT.F.27.M) was cultivated in fresh and saline water from their
respective media, namely Potato Dextrose Agar (PDA), Malt Extract Agar (MEA),
Potato Dextrose Broth (PDB), and Malt Extract Broth (MEB). The metabolites in
mycelia and agar were extracted using methanol, while ethyl acetate was used to
extract the metabolites in the broth. The crude extract was tested for
antimicrobials against M. luteus, B. subtillis, P. aeruginosa, C. albicans, and C.
tropicals. Meanwhile, fungal metabolites were characterized by phytochemical
tests, Thin Layer Chromatography (TLC), and Gas Chromatography-Mass
Spectrometer (GC-MS). The antimicrobial activity assay showed that PBt-M was
the most effective antimicrobial against Micrococcus luteus (Minimum Inhibition
Concentration (MIC) 6.25 µg/ml, Minimum Bactericidal Concentration (MBC)
12.5 µg/mL) and Bacillus subtilis (MIC 1.56 µg/mL; MBC 1.56 µg/mL).
Metabolite characterization for extract PBt-M using phytochemical tests and TLC
plate analysis indicated the presence of alkaloids and phenols. In contrast, the
results of GC-MS analysis successfully revealed the presence of compounds such
as trilaurin, vinyl laurate, and dodecanamide, N-dodecyl-N-(trifluoroacetyl).
Keywords: antibacterials; fungi; Gloniopsis; mangroves,

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