KUSUMA, NARITA RECI (2023) PENGARUH PERENDAMAN DALAM CHITOSAN NANOPARTICLES (CNPs) DAUN BIDARA ARAB (Ziziphus spina-christi) TERHADAP KELULUSHIDUPAN DAN PROFIL DARAH IKAN PATIN (Pangasius sp.) YANG DIINFEKSI Aeromonas hydrophila (23da47). Undergraduate thesis, Fakultas Perikanan dan Ilmu Kelautan.

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Abstract

ABSTRAK
profil darah.
(Narita Reci Kusuma, 26020118130051, Pengaruh Perendaman Chitosan
Nanoparticles (CNPs) Daun Bidara Arab (Ziziphus spina-christi) terhadap
Kelulushidupan dan Profil Darah Ikan Patin (Pangasius sp.) yang diinfeksi
Aeromonas hydrophila, Desrina & Sarjito)
Permasalahan yang seringkali ditemui pada budidaya ikan patin adalah
infeksi penyakit Motile Aeromonas Septicemia (MAS). Penyakit tersebut dapat
disebabkan oleh bakteri Aeromonas hydrophila. Namun, pengobatan A. hydrophila
masih mengandalkan antibiotik yang tidak ramah lingkungan, menimbulkan
resistensi bakteri, dan residu pada ikan apabila digunakan secara terus menerus.
Oleh karena itu diperlukan alternatif pengobatan penyakit MAS yang ramah
lingkungan dan aman bagi ikan, salah satunya Chitosan Nanoparticles (CNPs) daun
bidara arab. Penelitian ini bertujuan untuk mengkaji pengaruh CNPs daun bidara
arab terhadap kelulushidupan dan profil darah Pangasius sp. yang diinfeksi bakteri
A. hydrophila dengan metode perendaman. Penelitian menggunakan metode
eksperimental dengan model Rancangan Acak Lengkap dengan 5 perlakuan dan 3
kali ulangan. Dosis perendaman pada tiap perlakuan, yaitu A (0 ppm), B (450 ppm),
C (500 ppm), D (550 ppm) dan kontrol positif berupa tetrasiklin (10 ppm). Setelah
dilakukan perendaman dengan waktu pemeliharaan selama 14 hari, dilakukan
perhitungan pada tingkat kelulushidupan (SR), total leukosit, diferensial leukosit,
total eritrosit, hemoglobin (Hb), pertumbuhan bobot mutlak, laju pertumbuhan
relatif dan pengamatan gejala klinis.
Hasil yang didapatkan menunjukkan bahwa ikan patin yang telah diinfeksi
A. hydrophila menunjukkan gejala klinis berupa penurunan nafsu makan, ulcer
(borok), bercak merah, eksoptalmia, pigmentasi warna kulit, dan sirip geripis. Hasil
perendaman CNPs daun bidara arab tidak berpengaruh nyata (P>0,05) terhadap
kelulushidupan (SR), total leukosit, diferensial leukosit, total eritrosit, hemoglobin
(Hb), pertumbuhan bobot mutlak, laju pertumbuhan relatif. Namun berpengaruh
signifikan (P<0,05) pada persentase limfosit dan neutrofil. Secara keseluruhan,
hasil terbaik perendaman menggunakan CNPs daun bidara arab pada ikan patin
yang diinfeksi A. hydrophila pada perlakuan C (500 ppm) dengan kelulushidupan
tertinggi sebesar 46,7%.
Kata kunci: A. hydrophila, CNPs daun bidara arab, kelulushidupan, Pangasius sp.,

ABSTRACT
The problem often encountered in catfish farming is Motile Aeromonas
Septicemia (MAS) disease. The disease can be caused by the bacteria of Aeromonas
hydrophila. A. hydrophila handling still relies on the role of antibiotics which are
not environmentally friendly and if used continuously will cause residues in fish
and bacterial resistance. Therefore, an alternative treatment for MAS disease is
needed that is environmentally friendly and safe for fish, one of which is by
Chitosan Nanoparticles (CNPs) Sidr leaves. This research aims to study the effect
of the immersion CNPs of Sidr leave on the survival rate and blood profile of
Pangasius sp. infected with A. hydrophila of the test fish. The study used an
experimental method with a completely randomized design with 5 treatments and 3
replications. The dose for the test in each treatment, namely A (0 ppm), B (450
ppm), C (500 ppm), D (550 ppm), and positive control in the form of tetracycline
(10 ppm). After immersion for 14 days, survival rate (SR), total leukocytes,
leukocyte differential, total erythrocytes, hemoglobin (Hb), absolute weight growth,
relative growth rate (RGR), and observation of clinical symptoms were calculated.
The results obtained indicated that the catfish had been infected by A.
hydrophila showing some clinical symptoms such as decreased appetite, ulcers, red
patches, exophthalmia, skin color pigmentation, and fin flaking. The results of
CNPs Sidr leaves immersion had no significant effect (P>0.05) on survival rates
(SR), total leukocytes, differential leukocytes, total erythrocytes, hemoglobin (Hb),
absolute weight growth, and relative growth rate (RGR). However, it significantly
affects (P<0,05) the percentage of lymphocytes and neutrophils. Overall, the best
immersion results using CNPs of Sidr leaves in infected catfish A. hydrophila in
treatment C (500 ppm) with the highest survival rate of 46.7%.
Keywords: A. hydrophila, CNPs of Sidr leaves, Pangasius sp., survival rate, blood
profile.

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