HASNA, SALMA KHOIRONNIDA (2022) PENGOBATAN IKAN MAS (Cyprinus carpio), YANG TERINFEKSI Aeromonas hydrophila, DENGAN PERENDAMAN MENGGUNAKAN EKSTRAK LIDAH BUAYA (Aloe vera) TERHADAP PROFIL DARAH, PERTUMBUHAN, DAN KELANGSUNGAN HIDUPNYA (22b269). Undergraduate thesis, Fakultas Perikanan dan Ilmu Kelautan.

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Abstract

RINGKASAN
Salma Khoironnida Hasna. 26020117140082. Pengobatan Ikan Mas (Cyprinus
carpio), yang Terinfeksi Aeromonas hydrophila, dengan Perendaman
Menggunakan Ekstrak Lidah Buaya (Aloe vera) terhadap Profil Darah,
Pertumbuhan, dan Kelangsungan Hidupnya (Slamet Budi Prayitno dan Desrina)
Ikan mas (C. carpio) yang mengalami infeksi A. hydrophila dalam sistem
budidaya, perlu diberikan pengobatan hingga ikan sembuh, namun pengobatan
dengan bahan kimia dikhawatirkan dapat membahayakan lingkungan budidaya
dan membahayakan konsumen yang mengonsumsi ikan tersebut. Oleh karena itu,
diperlukan pengobatan dengan menggunakan bahan alami yang lebih ramah
lingkungan, dapat menghambat pertumbuhan bakteri, serta efektif menyembuhkan
penyakit MAS. Lidah buaya (A. vera) digunakan sebagai obat karena memiliki
aktivitas antibakteri yang diharapkan mampu mengobati ikan mas dari infeksi A.
hydrophila. Tujuan penelitian ini adalah untuk mengetahui kemampuan daya
hambat ekstrak lidah buaya terhadap serangan bakteri A. hydrophila secara in
vitro, mengetahui pengaruh perendaman ekstrak lidah buaya (A. vera) terhadap
jumlah konsentrasi A. hydrophila, profil darah, pertumbuhan, dan kelulushidupan
ikan mas (C. carpio) yang terinfeksi A. hydrophila, serta mengetahui efek dosis
ekstrak lidah buaya yang berbeda terhadap jumlah bakteri, profil darah,
pertumbuhan, dan kelulushidupan ikan mas. Metode penelitian yang digunakan
adalah metode eksperimental menggunakan rancangan acak lengkap (RAL)
dengan 4 perlakuan dan 3 ulangan. Perlakuan yang diujikan adalah perendaman
ikan mas dengan ekstrak lidah buaya dosis A (0 ppm), B (250 ppm), C (500 ppm),
dan D (750 ppm). Data yang dikumpulkan meliputi ukuran zona hambat ekstrak
lidah buaya, gejala klinis, jumlah A. hydrophila, profil darah, bobot mutlak, SGR,
kelulushidupan, dan kualitas air. Ekstrak lidah buaya menghasilkan diameter zona
hambat berkisar antara 7,33-11,02 mm. Hasil uji F (ANOVA) menunjukkan
bahwa perlakuan pra perendaman dengan ekstrak lidah buaya tidak berpengaruh
nyata terhadap jumlah bakteri dan profil darah. Perlakuan setelah perendaman,
berpengaruh nyata terhadap jumlah bakteri yang diduga A. hydrophila, total
eritrosit, total leukosit, kadar hemoglobin, kadar hematokrit, bobot mutlak, dan
SGR ikan mas, namun tidak berpengaruh nyata terhadap kelulushidupan ikan mas.
Hasil uji Dunnet menunjukkan dosis B (250 ppm), C (500 ppm), dan D (750 ppm)
berbeda nyata dengan A (0 ppm) pada perhitungan jumlah bakteri dengan rata-rata
A, B, C, dan D yakni 11,93±1,55; 8,53±1,00; 5,20±1,57; dan 4,27±0,57 CFU/ml,
hemoglobin dengan rata-rata A 7,20±0,40; B 8,67±0,46; C 11,80±0,35; dan D
12,20±0,20 g/dl, dan hematokrit dengan rata-rata A 19,00±2,00; B 26,33±2,08; C
31,67±2,08; dan D 36,33±1,53%. Perlakuan C dan D berbeda nyata dengan A
pada perhitungan leukosit dengan rata-rata A 5,64±0,22; C 3,24±0,86; dan D
1,51±0,71 sel/mm3
, bobot mutlak dengan rata-rata A 1,03±0,08; C 1,67±0,30; dan
D 1,88±0,17 g, dan SGR dengan rata-rata A 0,64±0,07; C 1,02±0,11; dan D
1,17±0,21 %/hari. Perlakuan D berbeda nyata dengan A pada perhitungan eritrosit
dengan rata-rata A dan D yakni 1,98±0,52 dan 2,84±0,15 sel/mm3
.
Kata kunci: Ikan Mas, Lidah Buaya, A. hydrophila, Perendaman

SUMMARY
Salma Khoironnida Hasna. 26020117140082. Treatment of Common Carp
(Cyprinus carpio), that Infected by Aeromonas hydrophila, by Immersion Using
Aloe vera Extract on Its Blood Profile, Growth, and Survival Rate (Slamet Budi
Prayitno and Desrina)
Common carp (C. carpio) that infected by A. hydrophila in the culture
system, need to be given treatment until the fish recover, but treatment with
chemicals is worried, since they can harm the culture environment and endanger
consumers who consume the fish. Therefore, required treatment using natural
ingredients that are more environmentally friendly, can inhibit bacterial growth,
and effectively cure MAS disease. Aloe vera is used as a medicine because it has
antibacterial activity which is expected to be able to treat common carp from A.
hydrophila infection. The purpose of this study is to determine the inhibitory
ability of aloe vera extract against the attack of A. hydrophila bacteria in vitro, to
determine the effect of soaking C. carpio in the aloe vera extract on the total
concentration of A. hydrophila, blood profile, growth, and survival rate of
common carp (C. carpio) that infected by A. hydrophila, and to determine the
effect of different doses of aloe vera extract on the number of bacteria, blood
profile, growth, and survival rate of common carp. The research method that was
used is an experimental method using a completely randomized design (CRD)
with 4 treatments and 3 replications. The treatments tested were immersion of
common carp in the aloe vera extract at doses of A (0 ppm), B (250 ppm), C (500
ppm), and D (750 ppm). The data collected included the size of the inhibition zone
of aloe vera extract, clinical symptoms, number of A. hydrophila, blood profile,
absolute weight value, SGR, survival rate, and water quality. Aloe vera extract
produce inhibition zone diameters that ranged from 7,33-11,02 mm. The results of
the F test (ANOVA) showed that the pre-soaking treatment with aloe vera extract
had no significant effect on the number of bacteria and blood profile. Immersion
of common carp in the aloe vera extract had a significant effect on the number of
bacteria suspected to be A. hydrophila, total erythrocytes, total leukocytes,
hemoglobin levels, hematocrit levels, absolute weight value, and SGR of common
carp, but did not significantly affect the survival rate of common carp. The results
of the Dunnet test showed that treatment B (250 ppm), C (500 ppm), and D (750
ppm) had significant differences with A (0 ppm) on the calculation of the number
of bacteria which the average of A, B, C, and D are 11.93±1.55; 8.53±1.00;
5.20±1.57; and 4.27±0.57 CFU/ml, hemoglobin which A is 7.20±0.40; B
8.67±0.46; C 11.80±0.35; and D 12.20 ± 0.20 g/dl, and hematocrit which A is
19.00 ± 2.00; B 26.33±2.08; C 31.67±2.08; and D 36.33±1.53%. Treatments C
and D had significant differences with A on leukocyte count which A is 5.64±0.22;
C 3.24±0.86; and D 1.51±0.71 cells/mm3
, absolute weight value which A is
1.03±0.08; C 1.67±0.30; and D 1.88±0.17 g, and SGR which A is 0.64±0.07; C
1.02±0.11; and D 1.17±0.21%/day. Treatment D had a significant difference with
A in the erythrocyte count which A is 1.98±0.52 and D 2.84±0.15 cells/mm3
.
Key words: Common Carp, Aloe Vera, A. hydrophila, Immersion

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