WIDYANTI, Trisna and Sutiningsih, Dwi and Hadi, Mochamad (2023) AKTIVITAS NANOKITOSAN EKSTRAK PASAK BUMI (Erycoma Longifolia Jack) SEBAGAI IMUNOMODULATOR TERHADAP INFEKSI Salmonella typhi (Studi Aktivitas Fagositosis Makrofag pada Hewan Uji Secara Invivo). Masters thesis, UNIVERSITAS DIPONEGORO.

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Abstract

Latar Belakang : Demam tifoid saat ini masih mengancam masyarakat diwilayah subtropis dan tropis terutama di Indonesia. Oleh karena itu perlu dikembangkannya terapi adjuvant dari bahan alami seperti Eurycoma longifolia Jack. Berdasarkan studi literatur masyarakat telah memanfaatkan Eurycoma longifolia Jack untuk menambah stamina.
Tujuan : Mengetahui aktivitas ekstrak Eurycoma longifolia Jack dan sediaan nanokitosannya terhadap aktivitas fagositosis makrofag secara invivo.
Metode : Jenis penelitian Quasi-experiment dengan Post-test only control group design. Pengujian imunomodulator ini diukur aktivitas fagositosis makrofag yaitu kapasitas fagositosis dan indeks fagositosis dihitung jumlah sel dengan pengamatan mikroskopis.
Hasil : Hasil sediaan nanokitosan ekstrak Eurycoma longifolia Jack berpotensi sebagai imunomodulator. Uji Post Hoc LSD menunjukan nilai p < 0,05 hal ini mendukung dosis 300 mg/kgBB bahwa semakin tinggi dosis maka dapat menggambarkan efek dari ekstrak dan nanokitosan Eurycoma longifolia Jack.
Kesimpulan : Ekstrak Eurycoma longifolia Jack dan sediaan nanokitosan ekstrak Eurycoma longifolia Jack memiliki aktivitas fagositosis makrofag secara in vivo, yaitu berpotensi sebagai efek imunomodulator. Hasil indeks fagositosis mampu meningkatkan aktivitas kekebalan tubuh (imunostimulan) dan menekan sistem kekebalan tubuh (imunosupresan).
Keyword : Nanokitosan, Eurycoma longifolia Jack, Imunomodulator

Background: Typhoid fever is still a threat to people in the subtropics and tropics, especially in Indonesia. Therefore, it is necessary to develop adjuvant therapy from natural ingredients such as Eurycoma longifolia Jack. Based on literature studies, the community has used Eurycoma longifolia Jack to increase stamina.
Objective : The objektif of this study is to determine activity of Eurycoma longifolia Jack extract and its nanochitosan preparation on macrophage phagocytosis activity invivo.
Method: This was an Quasi-experimental study using the Post-test only control group design. This immunomodulator test measured the phagocytosis activity of macrophages, namely phagocytosis capacity and phagocytosis index, which counted the number of cells by microscopic observation.
Results: The results of nanochitosan preparations of Eurycoma longifolia Jack extract have potential as immunomodulator. The LSD Post Hoc Test showed a p value < 0.05, this supports the dose of 300 mg/kgBB, that the higher the dose, the more the effect of the extract and nanochitosan Eurycoma longifolia Jack can be described.
Conclusion: Eurycoma longifolia Jack extract and nanochitosan preparation of Eurycoma longifolia Jack extract have macrophage phagocytosis activity invivo, which has the potential as an immunomodulatory effect. The results of the phagocytosis index can increase immune activity (immunostimulants) and suppress the immune system (immunosuppressants).
Keyword : Nanochitosan, Eurycoma longifolia Jack, Immunomodulator

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