FITRIANI, CICIH (2022) AKTIVITAS ANTIBAKTERI JAMUR EPIFIT DARI RUMPUT LAUT Padina sp. TERHADAP Vibrio parahaemolyticus (22ik581). Undergraduate thesis, Universitas Diponegoro.

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Abstract

ABSTRAK
Cicih Firiani. 26 040 118 130 091. Aktivitas Antibakteri dari Rumput Laut Padina
sp. Terhadap Vibrio parahaemolyticus (Subagiyo dan Ali Djunaedi)
Udang merupakan salah satu komoditas yang potensial di Indonesia, namun
masalah terjadi pada pembudidayaan udang yang mengalami kendala salah satunya
di akibatkan oleh serangan penyakit, dengan demikian perlunya pengobatan infeksi
yang di sebabkan oleh bakteri V. parahaemolyticus. Pemanfaatan mikroorganisme
yang dapat dimanfaatkan sebagai agen hayati adalah jamur epifit. Pemilihan jamur
laut dari rumput laut karena memiliki kandungan metabolit sekunder, salah satunya
sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri
dari ekstrak jamur epifit Padina sp. terhadap bakteri Vbirio parahaemolyticus.
Penelitian ini menggunakan metode eksperimental laboratoris dan uji aktivitas
antibakteri dengan rancangan acak lengkap (RAL), penelitian di lakukan dalam 7
tahapan metode, yaitu pengambilan sampel, isolasi jamur, skrining aktivitas
antibakteri jamur epifit Padina sp., ekstraksi, uji antibakteri ekstrak jamur epifit
Padina sp.,Uji fitokimia dan identifikasi jamur epifit Padina sp. Hasil Penelitian
menunjukan bahwa penapisan fitokimia dari ekstrak jamur Padina sp. menyatakan
bahwa ekstrak jamur tersebut di duga memiliki golongan senyawa alkaloid,
polifenol, flavonoid, dan terpenoid. Uji antibakteri ekstrak jamur Padina sp.
terhadap bakteri Vibrio parahaemolyticus di dapatkan hasil, yaitu pada konsentrasi
1000 µg/disk adalah 1,75 cm – 5,02 cm, konsentrasi 500 µg/disk berkisar 1,45 cm
– 4,44 cm, dan konsentrasi 250 µg/diks berkisar 1,94 cm – 4,18 cm. Berdasarkan
hasil penelitian dapat disimpulkan bahwa Esktrak jamur epifit dari rumput laut
Padina sp. memiliki aktivitas antibakteri terhadap bakteri Vibrio parahaemolyticus
dan diduga jenis jamur epifit dari rumput laut Padina sp. adalah Sclerotium sp.,
Aspergillus sp., Penicillium sp., Mucor sp., dan Trichoderma sp.
Kata Kunci: antibakteri, jamur laut, V. parahaemolyticus, Padina sp.., Metabolit
sekunder

ABSTRACT
Cicih Firiani. 260 401 181 300 91. Antibacterial Activity of Padina sp. Seaweed
Against Vibrio parahaemolyticus (Subagiyo dan Ali Djunaedi)
Shrimp is one of the potential commodities in Indonesia, but problems occur in
shrimp cultivation which is experiencing problems, one of which is caused by
disease, thus the need for treatment of infections caused by V. parahaemolyticus
bacteria. Utilization of microorganisms that can be used as biological agents is
epiphytic fungi. The selection of sea mushrooms from seaweed because they contain
secondary metabolites, one of which is as an antibacterial. This study aims to
determine the antibacterial activity of the extract of the epiphytic mushroom Padina
sp. against the bacterium V. parahaemolyticus. This study used laboratory
experimental methods and antibacterial activity tests with a completely randomized
design (CRD). The research was conducted in 7 stages of the method, namely
sampling, mushroom isolation, screening for the antibacterial activity of the Padina
sp. epiphytic fungus extract, extraction, antibacterial test of Padina sp. epiphytic
mushroom extract , Phytochemical test and identification of the epiphytic fungus
Padina sp. The results showed that the phytochemical screening of Padina sp.
stated that the mushroom extract was thought to have a class of alkaloid
compounds, polyphenols, flavonoids, and terpenoids. Antibacterial test of Padina
sp. mushroom extract. against V. parahaemolyticus bacteria the results were
obtained, namely at a concentration of 1000 µg/disk it was 1.75 cm – 5.02 cm, a
concentration of 500 µg/disk was around 1.45 cm – 4.44 cm, and a concentration
of 250 µg/disk was around 1 .94cm – 4.18cm. Based on the results of the study it
can be concluded that the epiphytic mushroom extract from Padina sp. has
antibacterial activity against V. parahaemolyticus bacteria and is suspected to be
a type of epiphytic fungus from seaweed Padina sp. are Sclerotium sp., Aspergillus
sp., Penicillium sp., Mucor sp., and Trichoderma sp..
Keywords: antibacterial, marine fungus, V. parahaemolyticus, Padina sp..,
Secondary metabolites

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