ABDULAZIZ, KHALID M. A. and Kisdjamiatun, Kisdjamiatun and Kristina, Tri Nur (2015) THE EFFECTIVITY ANNONA MURICATA TO REDUCE MIF LEVEL AND IMPROVE ERYTHROCYTE NUMBER (STUDY IN CEREBERAL MALARIA PHASE OF SWISS MICE). Masters thesis, Universitas Diponegoro.

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Abstract

BACKGROUND: Severe malaria by parasites Plasmodium falciparum is a vast majority of malaria associated morbidity and mortality. MIF involve in the pathogenesis of malarial anemia because it inhibits erythropoiesis. Annona muricata at a dose of 200 mg/kg and 400 mg/kg may act as anti-inflammatory and antinosiseptik with reduced volume of exudate and leukocyte migration This research to prove Annona muricata can reduce MIF level and improve erythrocyte number in swiss albino mice inoculated with Plasmodium berghei ANKA.

METHOD: Study design was experimental study. 36 swiss mice which devided into 6 groups. K(-) is healthy mice, P1 and P2 were group without inoculated PbA with A. muricata dosage 100 and 150 mg/kg BW. K(+) is group with inoculated PbA, P3 and P4 were group with inoculated PbA and A. muricata dosage 100 and 150 mg/kg BW. Blood was taken from eyes mice for erythrocyte. Level of MIF measure with spleen culture.

RESULT: Mean of MIF level K(-): 29.64; P1: 39.05; P2: 62.27; K(+): 46.79; P3: 57.36; and P4: 36.09. Mean number of erythrocyte K(-): 9,775,000; P1: 9,288,000; P2: 9,900,000; K(+): 6,376,000; P3: 8,028,333; and P4: 7,548,333.

CONCLUSION: There is no significant reducing of MIF level and no significant increase erythrocyte count in those PbA inoculated swiss albino mice which are treated with Annona muricata Linn extract during cerebral malaria phase than PbA inoculated mice that are untreated. There is no difference of effective dose of Annona muricata Linn extract to increase erythrocyte count of swiss albino mice inoculated with PbA.

Keywords: Annona muricata, P. berghei ANKA, Erythrocyte, MIF.

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