Mohalhel, Ahmed Ali and Kristina, Tri Nur and Winarto, Winarto (2014) VCO REDUCE IL-6 AND CRP IN WISTAR RATS WITH HIGH FAT DIET. Masters thesis, Universitas Diponegoro.

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Abstract

Background: High fat diet is associated with body weight gain, which can lead to high blood lipid level especially low density lipoprotein and Triglycerides. Changes in the level of fat product from high fat diet lead to produce pro inflammatory cytokines, including TNF and IL-6. IL-6 stimulates the hepatic synthesis of CRP as inflammatory marker. VCO contains of biological active substance such as antioxidant, which have advantage in preventing the oxidation of LDL leading to regulate of inflammatory response. This research aims to prove that VCO can reduce IL6 and CRP in wistar rats with high fat diet (HFD).
Methods: The design of the study was Post-test Only Randomized Control Group Design. 30 Wistar rats were randomly allocated into five groups with 6 rats in each group. First group was given normal diet, the second group only HFD, the third group HFD and VCO 1ml /day, fourth group HFD and VCO 1.2ml /day, and fifth group HFD and VCO 2ml /day.This research was carried out for 8 weeks, then blood was drawn from the eye to measure IL-6 and CRP by using ELISA.
Result: serum level IL6 in ANOVA results indicate there is significant different of all group ( p <0.05). The post hoc showed the negative control significantly lower than those of rats treated with 1ml/day (p = 0.031) , 1.2m l/ day (p = 0.025), 2ml/day (p = 0.012) and positive control(p = 0.002). CRP serum test homogeneity showed non homogeneity (p=0.010). Kruskal-Wallis test. This analysis showed no significance difference of CRP levels all groups (p = 0.177). Conclusions: VCO at any doses studied could not lowered the IL-6 and CRP levels of HFD rats.
Keywords : VCO, HFD, IL-6, CRP, rats.

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