Mohamed, Abdalrahman and Soemantri, Soemantri and Bahrudin, Bahrudin (2014) Effect of Mesenchymal Stem Cells on TNF-α and IL-10 expression in Atherosclerotic lesion. Masters thesis, Universitas Diponegoro.

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Abstract

Background: Atherosclerosis is one of the leading causes of cardiovascular mortality and morbidity in the world. Mesenchymal stem cells (MSCs) may able to ameliorate atherosclerosis through increasing of IL-10 and reducing of TNF-α expression. However, the effect of MSCs on atheroscherotic vessel has never been reported.
Objective: To investigate the effect of MSCs administration on TNF-α and IL-10 expression and on atherosclerotic lesion of abdominal aorta.
Method: Sprague Dawley Rats were divided into negative control group (fed a normal diet, n=6), positive control group (fed a high fat diet, n=6) and MSCs treatment group (fed a high fat diet and treated single dose of 5x106 MSCs, n=8). Atherosclerotic appearance in abdominal aorta wall was stained by hematoxylin and eosin, while IL-10 and TNF-α protein expression were observed by immunohistochemistry.
Results: Atherosclerotic appearance in the MSCs treated group was significant lower than in that of untreated group (p=0.007). TNF-α expression in macrophage was significant higher in the MSCs treated group than that of untreated group (p=0.047), while expression of IL-10 in tunica media and endothelial cells were not significant.
Conclusion: MSCs have a potential effect in ameliorating atherosclerotic lesion in SD rats mediated in part by reducing of TNF-α expression in macrophage of abdominal aorta, but not mediated by IL-10 expression.
Keywords: Mesenchymal stem cells, atherosclerosis, Sprague Dawley rats, TNF-α, IL-10.

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