Abdurahman, Amer and Suromo, Lisyani and Kisdjamiatun, Kisdjamiatun (2014) THE EFFECT OF EXTRA VIRGIN OLIVE OIL ON FOAM CELLS AND TNF-α IN HIGH FAT DIET WISTAR RATS. Masters thesis, Universitas Diponegoro.

	Text (Cover) Cover.pdf Download (271kB)
	Text (Chapter 1) Chapter 1.pdf Download (111kB)
	Text (Chapter 2) Chapter 2.pdf Restricted to Registered users only Download (160kB)
	Text (Chapter 3) Chapter 3.pdf Restricted to Registered users only Download (71kB)
	Text (Chapter 4) Chapter 4.pdf Restricted to Registered users only Download (170kB)
	Text (Chapter 5) Chapter 5.pdf Restricted to Registered users only Download (376kB)
	Text (Chapter 6) Chapter 6.pdf Restricted to Registered users only Download (71kB)
	Text (Chapter 7) Chapter 7.pdf Restricted to Registered users only Download (62kB)
	Text (References) References.pdf Restricted to Registered users only Download (83kB)
	Text (Appendixes) Appendixes.pdf Restricted to Registered users only Download (837kB)

Abstract

Background: Deleterious effects of HFD are an increase TNF- α levels and foam cells on animal model. Anti-inflammatory effect of EVOO might be beneficial in HFD rats. It was expected that EVOO reduced TNF- α levels and the presence of foam cells of HFD rats.
Objective : to investigate effect of EVOO in reducing TNF- α levels and the presence of foam cells of HFD rats.
Methods : This study used 30 rats novergicus strain wistar. Rats were allocated into 2 control groups and 3 treatment groups. Negative control group received standard diet and positive control group received HFD. Treatment groups received HFD and EVOO doses for 8 weeks. Then rats killed, (TNF-α) measured by (ELISA) and foam cells by using histopathological method.
Results: Serum TNF- levels of positive control, negative control and treatment groups are 3.71 ± 91.41 (mean SD) pg/ml, 2.43 ± 159.98, 3.84 ± 112.56, 3.26 ± 55.23, and 2.46 ± 39.37, respectively. Kruskal-wallis test showed no significant different of TNF- levels among 5 groups (p=0.070). The presence of foam cells found in positive control 6 (100%), negative control 0 (0%),while in treated groups were 5 (83.3%), 2 (33.3%), and 2 (40%) respectively. X2 test showed significant different among all groups (p = 0.004), among positive control and treated groups (p = 0.043), among negative control and treated groups (p = 0.031), but among treated groups (p = 0.175).
Conclusion: EVOO might play a minor effect in reducing TNF- α levels. EVOO at any dose studied is able to reducing the presence of foam cells.
Key Words: Extra virgin olive oil, high fat diet, TNF-α and foam cells.

Actions (login required)

View Item