LANINA, LANINA (2022) PENGARUH EKSTRAK DAUN BANDOTAN (Ageratum conyzoides) SEBAGAI ANESTESI DALAM PROSES TRANSPORTASI TERTUTUP TERHADAP KELULUSHIDUPAN BENIH KAKAP PUTIH (Lates calcarifer) (22B553). Undergraduate thesis, Universitas Diponegoro.

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Abstract

ABSTRAK
Lanina. 26020118130069. Pengaruh Ekstrak Daun Bandotan (Ageratum
conyzoides) Sebagai Anestesi Dalam Prooses Transportasi Tertutup Terhadap
Kelulushidupan Benih Kakap Putih (Lates calcarifer) (Sri Hastuti dan Tita
Elfitasari)
Transportasi benih merupakan kegiatan penting dalam kegiatan budidaya,
terutama apabila letak lokasi budidaya berjauhan dengan panti benih. Pemanfaatan
bahan pembius lokal seperti daun bandotan (Ageratum conyzoides) dilakukan untuk
menekan metabolisme ikan sehingga ikan tidak mudah mengalami stres dan
kematian selama kegiatan transportasi berlangsung. Tujuan dari penelitian ini
adalah mengetahui pengaruh ekstrak daun bandotan (Ageratum conyzoides) sebagai
bahan anestesi terhadap kelulushidupan benih kakap putih (Lates calcarifer) dalam
proses transportasi tertutup serta mengetahui dosis terbaik ekstrak daun bandotan
sebagai anestesi benih kakap putih dalam proses transportasi tertutup.
Penelitian ini bertujuan untuk mengetahui efek ekstrak daun bandotan
sebagai bahan anestesi dalam kegiatan transportasi terhadap imotilisasi dan
pengaruhnya terhadap kelulushidupan benih kakap putih. Penelitian ini
dilaksanakan pada tanggal 22 Mei – 13 Juni 2022 di Balai Besar Perikanan
Budidaya Air Payau (BBPBAP) Jepara tepatnya di hatchery unit pembenihan ikan
kakap putih.
Penelitian ini menggunakan metode eksperimental dan menggunakan uji
duncan dengan rancangan acak lengkap (RAL) 5 perlakuan dan 3 ulangan.
Perlakuan penambahan ekstrak daun bandotan sebagai anstesi yaitu A= 0 ml/L, B=
1 ml/L, C= 2 ml/L, D= 3 ml/L, E= 4 ml/L. Ikan uji yang digunakan adalah benih
kakap putih ukuran 8-10 cm dengan bobot 6-8 gram/ekor. Total ikan uji yang
digunakan berjumlah 300 ekor yang setiap packingnya (4L) berjumlah 20 ekor.
Data yang diamati meliputi tingkah laku ikan selama imotilisasi, lama waktu
induksi, lama waktu sedatif, kelulushidupan, dan kualitas air sebelum dan sesudah
transportasi.
Penambahan ekstrak daun bandotan sebagai bahan anestesi memberikan
pengaruh nyata (P<0,05) terhadap lama waktu induksi, lama waktu sedatif, dan
kelulushidupan benih kakap putih. Pemberian ekstrak daun bandotan menghasilkan
waktu induksi tercepat pada perlakuan E (4 ml/L) dengan rata-rata lama waktu
induksi 14,62±0,36 menit. Perlakuan B (1 ml/L) dengan rata-rata lama waktu
sedatif 14,30±0,82 menit menghasilkan waktu sedatif tercepat, dan perlaakuan D (3
ml/L) dengan rata-rata kelulushidupan benih 91,66±2,88% menghasilkan
kelulushidupan tertinggi. Kualitas air sebelum dan sesudah transportasi masih
dalam kisaran optimal untuk transportasi benih kakap putih.
Kata Kunci: Daun bandotan, benih kakap putih, anestesi, kelulushidupan,
transportasI

ABSTRACT
Lanina. 26020118130069. Effect of Bandotan Leaf Extract (Ageratum conyzoides)
as Anesthesia in Closed Transport Processes on the Survival of White Snapper
(Lates calcarifer) Seeds (Sri Hastuti and Tita Elfitasari)
Seed transportation is an important activity in cultivation activities,
especially if the location of the cultivation location is far from the seed house.
Utilization of local anesthetics such as bandotan leaves (Ageratum conyzoides) is
carried out to suppress fish metabolism so that fish are not prone to stress and death
during transportation activities. The purpose of this study was to determine the
effect of bandotan leaf extract (Ageratum conyzoides) as an anesthetic agent on the
survival of barramundi (Lates calcarifer) seeds in the closed transportation process
and to determine the best dose of bandotan leaf extract as an anesthetic for
barramundi seeds in the closed transportation process.
This study aims to determine the effect of bandotan leaf extract as an
anesthetic in transport activities on immotilization and its effect on the survival of
white snapper seeds. This research was conducted on 22 May – 13 June 2022 at
the Center for Brackish Water Aquaculture Fisheries (BBPBAP) Jepara, precisely
in the hatchery of the white snapper hatchery unit.
This study used an experimental method and used Duncan's test with a
completely randomized design (CRD) with 5 treatments and 3 replications. The
addition of bandotan leaf extract as an anesthetic was A= 0 ml/L, B= 1 ml/L, C= 2
ml/L, D= 3 ml/L, E= 4 ml/L. The test fish used were white snapper seeds measuring
8-10 cm with a weight of 6-8 grams/head. The total test fish used were 300 fish,
each packing (4L) was 20 fish. The observed data included fish behavior during
immotilization, induction time, sedative time, survival rate, and water quality
before and after transportation.
The addition of bandotan leaf extract as an anesthetic agent had a
significant effect (P<0.05) on the length of induction time, length of sedative time,
and survival of white snapper seeds. The administration of bandotan leaf extract
resulted in the fastest induction time in treatment E (4 ml/L) with an average
induction time of 14.62±0.36 minutes. Treatment B (1 ml/L) with an average
sedative time of 14.30±0.82 minutes produced the fastest sedative time, and
treatment D (3 ml/L) with an average seed survival of 91.66±2.88. % resulted in
the highest survival rate. The water quality before and after transportation was still
in the optimal range for the transportation of white snapper seeds.
Keywords: Bandotan leaves, barramundi seeds, anesthesia, survival,
transportation

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