FIDIANTI, ANISA (2022) PENGARUH PENYUNTIKAN HORMON 17α-METILTESTOSTERON DENGAN DOSIS YANG BERBEDA TERHADAP PERFORMA REPRODUKSI INDUK LELE JANTAN (Clarias gariepinus Burchell)(22b215). Undergraduate thesis, Universitas Diponegoro.

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Abstract

RINGKASAN
Anisa Fidianti. 26020118130055. Pengaruh Penyuntikan Hormon 17α-Metiltestosteron dengan Dosis yang Berbeda Terhadap Peforma Reproduksi Induk Lele Jantan (Clarias gariepinus Burchell) (Fajar Basuki dan Tristiana Yuniarti)
Penyuntikan Hormon 17α-Metiltestosteron pada induk lele jantan (Clarias gariepinus Burchell) penting dilakukan untuk memperbaiki peforma reproduksi.
Penelitian ini bertujuan tujuan untuk mengetahui pengaruh penyuntikan hormon
17α-Metiltestosteron terhadap performa reproduksi dan mengetahui dosis terbaik terhadap kecepatan pematangan gonad induk lele jantan. Dosis optimum hormon 17α-Metiltestosteron akan memacu spermatogenesis sehingga pembetukan sperma berlangsung lebih cepat dan berkualitas. Penelitian ini berlangsung pada tanggal 15 Maret – 25 April 2022 di Teaching Factory Undip. Penelitian ini menggunakan metode eksperimental dengan Rancangan Acak Lengkap (RAL) terdiri dari 4 perlakuan dan 3 ulangan yakni perlakuan A (0 mg 17α-Metiltestosteron /kg induk), perlakuan B (15 mg 17α-Metiltestosteron/kg induk), perlakuan C (21 mg 17α-Metiltestosteron/kg induk), dan perlakuan D (27 mg 17α-Metiltestosteron/kg induk). Indukan lele jantan dengan bobot 1-1,2kg digunakan sebagai ikan uji. Variabel yang diamati meliputi kecepatan pematangan gonad, histologi gonad, total
protein plasma, fertilization rate, hatcing rate, survival rate larva dan kualitas air. Hasil penelitian menunjukkan dosis terbaik yaitu perlakuan D (27 mg 17α-Metiltestosteron/kg induk) dengan kecepatan pematangan gonad selama 15±1 hari,
pengamatan histologi menunjukan gonad berada di TKG IV, terjadi peningkatan
kadar total protein plasma berkisar 3-5 g/dL, fertilization rate (FR) sebesar
89,2±2,02%, hatching rate (HR) sebesar 83,1±2,27%, dan survival rate (SR) larva
sebesar 81,83±1,26%. Hasil pengukuran kualitas air diperoleh suhu berkisar 26,6-28,8 ºC, pH berkisar 6,84 – 7,6, dan DO berkisar 3,1-5,3 mg/L. Hasil penelitian
menunjukan bahwa penyuntikan 17α-Metiltestosteron mampu memberi pengaruh
nyata terhadap performa reproduksi induk lele jantan dengan dosis terbaik 27 mg/kg
bobot tubuh.
Kata kunci : testosteron, kematangan gonad, reproduksi, spermatogenesis

SUMMARY
Anisa Fidianti. 26020118130055. The Effect of 17α-Methyltestosterone
Hormone Injection with Different Doses on Reproductive Performance of Male
Catfish Broodstock (Clarias gariepinus Burchell) (Fajar Basuki dan Tristiana
Yuniarti)
Injection of the hormone 17α-methyltestosterone in male catfish (Clarias
gariepinus Burchell) is important to improve reproductive performance. This study
aims to determine the effect of injection of the hormone 17α-methyltestosterone on
reproductive performance and to determine the best dose on the gonadal
maturation rate of male catfish. The optimum dose of 17α-Methyltestosterone
hormone will stimulate the process of spermatogenesis so therefore sperm
formation takes place more quickly and with higher quality. This research took
place from March 15 to April 25 2022 at the Teaching Factory of Undip. This
research used an experimental method with a completely randomized design (CRD)
consisting of 4 treatments and 3 replications. Treatment A (0 mg 17α-methyltestosterone/kg broodstock), treatment B (15 mg 17α-methyltestosterone/kg
broodstock), treatment C (21 mg 17α-Methyltestosterone/kg broodstock), and
treatment D (27 mg 17α- Methyltestosterone/kg broodstock). Male catfish
broodstock weighing 1-1.2 kg was used as research fish. The observed variables
included gonadal maturation rate, gonadal histology, plasm proteins total,
fertilization rate, hatching rate, the survival rate of larvae, and water quality. The
results showed that the best dose was treatment D (27 mg 17α-methyltestosterone/kg
broodstock) with a gonadal maturation rate of 15±1 days, histological
observations showed gonadal maturation occurred in TKG IV with an increase in
plasm proteins total levels ranging from 3-5 g/dL, fertilization rate (FR) of
89.2±2.02%, hatching rate (HR) of 83.1±2.27%, and survival rate (SR) of larvae
was 81.83±1.26%. The results of water quality measurements obtained during the
attempt displayed that temperatures ranged from
26.6 to 28.8 0 C, pH ranged from 6.84 to 7.6, and DO ranged from 3.1-5.3 mg/L.
The results showed that the injection of 17α-Methyltestosterone was able to have a
significant effect on the reproductive performance of male catfish with the best dose
of 27 mg/kg body weight.
Key word : testosterone, gonadal maturation, reproduction, spermatogenesis

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